LymphedemaV1, Main, Exploration, bibRecord, 004106

Molecular mimicry between cockroach and helminth glutathione S-transferases promotes crossreactivity and cross-sensitization

Identifieur interne : 004106 ( Main/Exploration ); précédent : 004105; suivant : 004107

Molecular mimicry between cockroach and helminth glutathione S-transferases promotes crossreactivity and cross-sensitization

Auteurs : Helton Santiago [États-Unis] ; Elyse Leevan [États-Unis] ; Sasisekhar Bennuru [États-Unis] ; Flavia Ribeiro-Gomes [États-Unis] ; Ellen Mueller [États-Unis] ; Mark Wilson [États-Unis] ; Thomas Wynn [États-Unis] ; David Garboczi [États-Unis] ; Joseph Urban [États-Unis] ; Edward Mitre [États-Unis] ; Thomas B. Nutman

Source :

The Journal of Allergy and Clinical Immunology [ 0091-6749 ] ; 2012.

RBID : PMC:3387355

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies (blood), Cockroaches (enzymology), Cockroaches (genetics), Cockroaches (immunology), Cross Reactions, Elephantiasis, Filarial (immunology), Epitope Mapping, Female, Glutathione Transferase (chemistry), Glutathione Transferase (genetics), Glutathione Transferase (immunology), Helminth Proteins (chemistry), Helminth Proteins (genetics), Helminth Proteins (immunology), Helminths (enzymology), Helminths (immunology), Humans, Immunoglobulin E (blood), Immunoglobulin E (immunology), Insect Proteins (chemistry), Insect Proteins (genetics), Insect Proteins (immunology), Mice, Mice, Inbred BALB C, Models, Molecular, Molecular Mimicry (immunology), Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Trichostrongyloidea (immunology), Trichostrongyloidiasis (immunology), Wuchereria bancrofti (enzymology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
MESH :
- chemical , blood : Antibodies, Immunoglobulin E.
- chemical , chemistry : Glutathione Transferase, Helminth Proteins, Insect Proteins.
- enzymology : Cockroaches, Helminths, Wuchereria bancrofti.
- genetics : Cockroaches, Glutathione Transferase, Helminth Proteins, Insect Proteins, Wuchereria bancrofti.
- immunology : Cockroaches, Elephantiasis, Filarial, Glutathione Transferase, Helminth Proteins, Helminths, Immunoglobulin E, Insect Proteins, Molecular Mimicry, Trichostrongyloidea, Trichostrongyloidiasis, Wuchereria bancrofti.
- Amino Acid Sequence, Animals, Cross Reactions, Epitope Mapping, Female, Humans, Mice, Mice, Inbred BALB C, Models, Molecular, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA.

Abstract

Background

The extensive similarities between helminth proteins and allergens are thought to contribute to helminth-driven allergic sensitization.

Objective

To investigate the cross-reactivity between a major glutathione-S transferase (GST) allergen of cockroach (Bla g 5) and the GST of Wuchereria bancrofti (WbGST), a major lymphatic filarial pathogen of humans.

Methods

We compared the molecular and structural similarities between Bla g 5 and WbGST by in silico analysis and by linear epitope mapping. Levels of IgE, IgG and IgG4 antibodies were measured in filarial-infected and –uninfected patients. Mice were infected with Heligmosomoides bakeri (Hb) and skin tested for cross-reactive allergic responses.

Results

These two proteins are 30% identical at the amino acid level with remarkable similarity in the N-terminal region and overall structural conservation based on predicted three-dimensional models. Filarial infection was associated with IgE, IgG, and IgG4 anti-Bla g 5 Ab production, with a significant correlation between Abs (irrespective of isotype) to Bla g 5 and WbGST (P < 0.0003). Pre-incubation of sera from cockroach allergic subjects with WbGST partially depleted (by 50 to 70%) anti-Bla g 5 IgE, IgG, and IgG4 Abs. IgE epitope mapping of Bla g 5 revealed that two linear N-terminal epitopes are highly conserved in WbGST corresponding to Bla g 5 peptides partially involved in the inhibition of WbGST binding. Finally, mice infected with Hb developed anti-HbGST IgE and showed immediate type skin test reactivity to Bla g 5.

Conclusion

These data demonstrate that helminth GST and the aeroallergen Bla g 5 share epitopes that can induce allergic cross-sensitization.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387355

DOI: 10.1016/j.jaci.2012.02.045
PubMed: 22541242
PubMed Central: 3387355

Affiliations:

Le document en format XML

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<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Urban, Joseph" sort="Urban, Joseph" uniqKey="Urban J" first="Joseph" last="Urban">Joseph Urban</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
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<author><name sortKey="Mitre, Edward" sort="Mitre, Edward" uniqKey="Mitre E" first="Edward" last="Mitre">Edward Mitre</name>
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<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Nutman, Thomas B" sort="Nutman, Thomas B" uniqKey="Nutman T" first="Thomas B." last="Nutman">Thomas B. Nutman</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies (blood)</term>
<term>Cockroaches (enzymology)</term>
<term>Cockroaches (genetics)</term>
<term>Cockroaches (immunology)</term>
<term>Cross Reactions</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Epitope Mapping</term>
<term>Female</term>
<term>Glutathione Transferase (chemistry)</term>
<term>Glutathione Transferase (genetics)</term>
<term>Glutathione Transferase (immunology)</term>
<term>Helminth Proteins (chemistry)</term>
<term>Helminth Proteins (genetics)</term>
<term>Helminth Proteins (immunology)</term>
<term>Helminths (enzymology)</term>
<term>Helminths (immunology)</term>
<term>Humans</term>
<term>Immunoglobulin E (blood)</term>
<term>Immunoglobulin E (immunology)</term>
<term>Insect Proteins (chemistry)</term>
<term>Insect Proteins (genetics)</term>
<term>Insect Proteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Molecular</term>
<term>Molecular Mimicry (immunology)</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Sequence Analysis, DNA</term>
<term>Trichostrongyloidea (immunology)</term>
<term>Trichostrongyloidiasis (immunology)</term>
<term>Wuchereria bancrofti (enzymology)</term>
<term>Wuchereria bancrofti (genetics)</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Anticorps (sang)</term>
<term>Blattes (enzymologie)</term>
<term>Blattes (génétique)</term>
<term>Blattes (immunologie)</term>
<term>Cartographie épitopique</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Glutathione transferase ()</term>
<term>Glutathione transferase (génétique)</term>
<term>Glutathione transferase (immunologie)</term>
<term>Helminthes (enzymologie)</term>
<term>Helminthes (immunologie)</term>
<term>Humains</term>
<term>Immunoglobuline E (immunologie)</term>
<term>Immunoglobuline E (sang)</term>
<term>Infections à Trichostrongyloidea (immunologie)</term>
<term>Mimétisme moléculaire (immunologie)</term>
<term>Modèles moléculaires</term>
<term>Phylogénie</term>
<term>Protéines d'helminthes ()</term>
<term>Protéines d'helminthes (génétique)</term>
<term>Protéines d'helminthes (immunologie)</term>
<term>Protéines d'insecte ()</term>
<term>Protéines d'insecte (génétique)</term>
<term>Protéines d'insecte (immunologie)</term>
<term>Réactions croisées</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Séquence d'acides aminés</term>
<term>Trichostrongyloidea (immunologie)</term>
<term>Wuchereria bancrofti (enzymologie)</term>
<term>Wuchereria bancrofti (génétique)</term>
<term>Wuchereria bancrofti (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies</term>
<term>Immunoglobulin E</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glutathione Transferase</term>
<term>Helminth Proteins</term>
<term>Insect Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Blattes</term>
<term>Helminthes</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cockroaches</term>
<term>Helminths</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cockroaches</term>
<term>Glutathione Transferase</term>
<term>Helminth Proteins</term>
<term>Insect Proteins</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Blattes</term>
<term>Glutathione transferase</term>
<term>Protéines d'helminthes</term>
<term>Protéines d'insecte</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Blattes</term>
<term>Filariose lymphatique</term>
<term>Glutathione transferase</term>
<term>Helminthes</term>
<term>Immunoglobuline E</term>
<term>Infections à Trichostrongyloidea</term>
<term>Mimétisme moléculaire</term>
<term>Protéines d'helminthes</term>
<term>Protéines d'insecte</term>
<term>Trichostrongyloidea</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cockroaches</term>
<term>Elephantiasis, Filarial</term>
<term>Glutathione Transferase</term>
<term>Helminth Proteins</term>
<term>Helminths</term>
<term>Immunoglobulin E</term>
<term>Insect Proteins</term>
<term>Molecular Mimicry</term>
<term>Trichostrongyloidea</term>
<term>Trichostrongyloidiasis</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps</term>
<term>Immunoglobuline E</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cross Reactions</term>
<term>Epitope Mapping</term>
<term>Female</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Cartographie épitopique</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Glutathione transferase</term>
<term>Humains</term>
<term>Modèles moléculaires</term>
<term>Phylogénie</term>
<term>Protéines d'helminthes</term>
<term>Protéines d'insecte</term>
<term>Réactions croisées</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P2">The extensive similarities between helminth proteins and allergens are thought to contribute to helminth-driven allergic sensitization.</p>
</sec>
<sec id="S2"><title>Objective</title>
<p id="P3">To investigate the cross-reactivity between a major glutathione-S transferase (GST) allergen of cockroach (Bla g 5) and the GST of <italic>Wuchereria bancrofti</italic>
 (WbGST), a major lymphatic filarial pathogen of humans.</p>
</sec>
<sec id="S3"><title>Methods</title>
<p id="P4">We compared the molecular and structural similarities between Bla g 5 and WbGST by in silico analysis and by linear epitope mapping. Levels of IgE, IgG and IgG4 antibodies were measured in filarial-infected and –uninfected patients. Mice were infected with <italic>Heligmosomoides bakeri</italic>
 (Hb) and skin tested for cross-reactive allergic responses.</p>
</sec>
<sec id="S4"><title>Results</title>
<p id="P5">These two proteins are 30% identical at the amino acid level with remarkable similarity in the N-terminal region and overall structural conservation based on predicted three-dimensional models. Filarial infection was associated with IgE, IgG, and IgG4 anti-Bla g 5 Ab production, with a significant correlation between Abs (irrespective of isotype) to Bla g 5 and WbGST (<italic>P</italic>
 < 0.0003). Pre-incubation of sera from cockroach allergic subjects with WbGST partially depleted (by 50 to 70%) anti-Bla g 5 IgE, IgG, and IgG4 Abs. IgE epitope mapping of Bla g 5 revealed that two linear N-terminal epitopes are highly conserved in WbGST corresponding to Bla g 5 peptides partially involved in the inhibition of WbGST binding. Finally, mice infected with <italic>Hb</italic>
 developed anti-HbGST IgE and showed immediate type skin test reactivity to Bla g 5.</p>
</sec>
<sec id="S5"><title>Conclusion</title>
<p id="P6">These data demonstrate that helminth GST and the aeroallergen Bla g 5 share epitopes that can induce allergic cross-sensitization.</p>
</sec>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Maryland</li>
</region>
</list>
<tree><noCountry><name sortKey="Nutman, Thomas B" sort="Nutman, Thomas B" uniqKey="Nutman T" first="Thomas B." last="Nutman">Thomas B. Nutman</name>
</noCountry>
<country name="États-Unis"><region name="Maryland"><name sortKey="Santiago, Helton" sort="Santiago, Helton" uniqKey="Santiago H" first="Helton" last="Santiago">Helton Santiago</name>
</region>
<name sortKey="Bennuru, Sasisekhar" sort="Bennuru, Sasisekhar" uniqKey="Bennuru S" first="Sasisekhar" last="Bennuru">Sasisekhar Bennuru</name>
<name sortKey="Garboczi, David" sort="Garboczi, David" uniqKey="Garboczi D" first="David" last="Garboczi">David Garboczi</name>
<name sortKey="Leevan, Elyse" sort="Leevan, Elyse" uniqKey="Leevan E" first="Elyse" last="Leevan">Elyse Leevan</name>
<name sortKey="Mitre, Edward" sort="Mitre, Edward" uniqKey="Mitre E" first="Edward" last="Mitre">Edward Mitre</name>
<name sortKey="Mueller, Ellen" sort="Mueller, Ellen" uniqKey="Mueller E" first="Ellen" last="Mueller">Ellen Mueller</name>
<name sortKey="Ribeiro Gomes, Flavia" sort="Ribeiro Gomes, Flavia" uniqKey="Ribeiro Gomes F" first="Flavia" last="Ribeiro-Gomes">Flavia Ribeiro-Gomes</name>
<name sortKey="Urban, Joseph" sort="Urban, Joseph" uniqKey="Urban J" first="Joseph" last="Urban">Joseph Urban</name>
<name sortKey="Wilson, Mark" sort="Wilson, Mark" uniqKey="Wilson M" first="Mark" last="Wilson">Mark Wilson</name>
<name sortKey="Wynn, Thomas" sort="Wynn, Thomas" uniqKey="Wynn T" first="Thomas" last="Wynn">Thomas Wynn</name>
</country>
</tree>
</affiliations>
</record>

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Molecular mimicry between cockroach and helminth glutathione S-transferases promotes crossreactivity and cross-sensitization

Molecular mimicry between cockroach and helminth glutathione S-transferases promotes crossreactivity and cross-sensitization

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